SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析

doi:10.11843/j.issn.0366-6964.2015.10.006

摘要/Abstract

摘要：

旨在探讨SIRT1和SIRT2基因的多态性与秦川牛肉用性状的关系，寻找与秦川牛肉用性状相关的分子标记。本研究利用DNA 测序和PCR-PFLP方法检测秦川牛SIRT1和SIRT2基因的多态位点并对其进行基因分型，同时分析了单个多态位点不同基因型及合并基因型与秦川牛肉用性状的关联性。结果发现，在SIRT1基因3′侧翼区检测到两个多态位点（G25764A和A25846G），同样在SIRT2基因3′侧翼区也检测到两个突变位点（C19501T和C19518T）。关联性分析表明，在本试验所选取的505头秦川牛样本中，G25764A位点上AA基因型个体的眼肌面积极显著高于AB基因型（P＜0.01）；A25846G位点上AA基因型个体的眼肌面积与AB和BB两组基因型分别存在显著性差异（P＜0.05）和极显著性差异（P＜0.01）；C19501T位点的不同基因型对眼肌面积和肌间脂肪影响显著（P＜0.05），优良基因型为AA；C19518T的BB基因型个体背膘厚显著高于AA基因型（P＜0.05）。通过合并基因型发现，AB-BB-AA-BB的各个肉用性状表现最优（P＜0.01或P＜0.05）。以上结果提示，SIRT1和SIRT2基因单核苷酸多态性及合并基因型对秦川牛肉用性状有一定的影响，可以作为进行秦川牛肉用性状分子标记辅助选择的候选基因。

Abstract:

This study aimed to explore the association of single nucleotide polymorphism(SNP) of SIRT1 and SIRT2 genes with Qinchuan cattle meat quality traits，so as to search for molecular markers related with meat quality traits.DNA seuqencing and PCR-PFLP methods were applied to detect the genotype polymorphisms of SIRT1 and SIRT2 genes in Qinchuan cattle populations，and analyze the associations of single and combined genotypes with meat quality traits of Qinchuan cattle.Results showed that 2 SNPs(G25764A and A25846G) were detected in 3′-untranslated region of SIRT1 gene，and 2 mutants(C19501T and C19518T) in 3′-untranslated region of SIRT2 gene were identified，too.Association analysis indicated that，in Qinchuan cattle population with 505 individuals，at G25764A locus，individuals with AA genotype exhibited very significantly higher loin-eye area than those with AB genotype(P<0.01).At A25846G locus，the loin-eye area of animals with AA genotype was significantly higher than that of animals with AB genotype(P<0.05) and very significantly higher than that of animals with BB genotype(P<0.01).Moreover，the C19501T locus significantly affected loin-eye area and intramuscular fatty content(P＜0.05)，and AA was the preferable genotype.Besides，at C19518T locus，individuals with the BB genotype showed significantly higher backfat thinckness compared to those with the AA genotype(P<0.05).The optimal combined genotype of SIRT1-SIRT2 was AB-BB-AA-BB，which was more effective on meat quality traits than other combined genotypes(P＜0.01 or P＜0.05).These results suggested that the single nucleotide polymorphisms and combined genotypes of both SIRT1 and SIRT2 genes affected the meat quality traits of Qinchuan cattle，and they could be used as candidate genes for breeding Qinchuan cattle with better meat quality traits.

中图分类号:

S823
S813.3

桂林生，昝林森，王洪宝，王洪程. SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析[J]. 畜牧兽医学报, 2015, 46(10): 1741-1749.

GUI Lin-sheng，ZAN Lin-sen，WANG Hong-bao，WANG Hong-cheng. Effects of Single and Combined Genotypes of SIRT1 and SIRT2 Genes on Meat Quality Traits in Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1741-1749.

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https://www.xmsyxb.com/CN/Y2015/V46/I10/1741

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